Journal: International Journal of Nanomedicine

Article Title: Breakthrough of Hypoxia Limitation by Tumor-Targeting Photothermal Therapy-Enhanced Radiation Therapy

doi: 10.2147/IJN.S450124

Figure Lengend Snippet: Multi-modality imaging by PIBD. ( A ) In vitro CT contrast images and CT values of PIBD at different concentrations; ( B ) In vitro PA contrast images and PA intensity of PIBD at different concentrations; ( C ) In vitro NIRF contrast images and NIRF intensity of PIBD at different concentrations; ( D ) In vivo PA images of tumors in 4T1 tumor-bearing mice after i.v. injection of PIBD at different time points; ( E ) Changes of PA-signal intensity within tumor regions at corresponding time points; ( F ) In vivo CT images of tumors in 4T1 tumor-bearing mice after i.v. injection of PIBD at different time points; ( G ) Changes of CT value within tumor regions at corresponding time points; ( H ) In vivo NIRF images of tumors in 4T1 tumor-bearing mice after i.v. injection of PIBD at different time points; ( I ) Changes of NIRF fluorescence intensity within tumor regions at corresponding time points; ( J ) In vivo NIRF images of tumor and organs in 4T1 tumor-bearing mice after i.v. injection of PIBD at 24h.

Article Snippet: 4T1 cells (Mouse breast cancer cells) was purchased from Wuhan Punosai Life Technology Co., LTD.

Techniques: Imaging, In Vitro, In Vivo, Injection, Fluorescence

Journal: International Journal of Nanomedicine

Article Title: Breakthrough of Hypoxia Limitation by Tumor-Targeting Photothermal Therapy-Enhanced Radiation Therapy

doi: 10.2147/IJN.S450124

Figure Lengend Snippet: In vitro treatment. ( A ) Cell viability of 4T1 cells in different treatments by CCK8 assay; ( B ) Fluorescence intensity of DCFH-DA by different treatments; ( C ) Enhanced ROS production by PIBD in 4T1 cells. Confocal images (green fluorescence indicates positive staining for ROS stained with DCFH-DA) by different treatments (scale bar=100 μm); ( D ) DNA double strand breakage by PIBD in 4T1 cells. Confocal images (green fluorescence indicates positive staining for DNA double strand breakage stained with γ-H2AX) by different treatments (scale bar=10 μm). ( E ) Fluorescence intensity of γ-H2AX by different treatments; ( F ) Apoptosis rate of 4T1 cells in different treatments; ( G ) Apoptosis of 4T1 cells in different treatments by flow cytometry. (**P<0.01, ***P<0.001).

Article Snippet: 4T1 cells (Mouse breast cancer cells) was purchased from Wuhan Punosai Life Technology Co., LTD.

Techniques: In Vitro, CCK-8 Assay, Fluorescence, Staining, Flow Cytometry

Journal: Biosensors

Article Title: Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide–MXene–Multi-Walled Carbon Nanotubes Electrode

doi: 10.3390/bios14060261

Figure Lengend Snippet: Quantitative cell viability results by CCK−8 assay for ( A ) 4T1 and ( B ) MCF−7 cells incubated with the 3D rGO–Ti 3 C 2 –MWCNTs electrode for 0, 1, 2, 3, 4, and 5 h. ( C ) Reduction current response of the 3D rGO–Ti 3 C 2 –MWCNTs electrode with the addition of fMLP, catalase, and fMLP-free PBS to deoxygenated 0.01 M PBS solution containing 7.0 × 10 6 4T1 cells, 5.0 × 10 6 MCF−7 cells, and no cells. ( D ) Amperometric current response of the 3D rGO–Ti 3 C 2 –MWCNTs electrode after separate injections of fMLP, catalase, and fMLP-free PBS into breast cancer tissue immersed in the deoxygenated 0.01 M PBS solution.

Article Snippet: Human breast cancer cell line MCF−7 and mouse breast cancer cell line 4T1 were purchased from Servicebio Technology Co., Ltd. (Wuhan, China).

Techniques: CCK-8 Assay, Incubation